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Fig. S2 TetA-GBD tagged with p2a Cherry is functional. (A and B) YFP RNA expression detected by whole-mount in situ hybridization in TetRE:Axin1-YFP transgenic embryos injected with equimolar amounts of TetA-GBD (50 pg) or TetA-GBD p2a Cherry (65 pg), and treated with EtOH vehicle or 25 μg/mL Dox plus 100 μM Dex from 5 h postfertilization (hpf) for 3.5 h. (C) Quantification of data presented in A and B. Axin1-YFP RNA expression levels were classified into five classes, and the number of embryos in each class was counted.
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