(A) irx3a and nkx2.2a knockdown severely perturb hlxb9 expression in pancreatic endocrine cells. Representative confocal projections of 30 hpf hlxb9: GFP larvae injected with control morpholino (A–C), irx3a morpholino (D and E), and irx3a/nkx2.2a morpholinos (F, G, and H). (B) irx3a and nkx2.2a knockdown increases hb9 expression in spinal cord motor neurons (MNs). Representative confocal projections of 30 hpf hb9:GFP larvae injected with control morpholino (A), irx3a morpholino (B), and irx3a/nkx2.2a morpholinos (C).
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