ZFIN Image: Sarmah et al., 2010, Fig. 3

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Figure Caption

Fig. 3

IP6K2 is required for Hh target gene expression during development. (A–J) Whole-mount in situ hybridizations showing expression of Hh target genes in zebrafish embryos (24-hpf stage) injected with either control MO (column 1) or ip6k2^SPMO (column 2). Expression of shh (A–D) was slightly elevated, whereas ptc1 (E–H) and gli1 (I and J) expression was down-regulated in ip6k2^SPMO (B, D, F, H, and J) compared with controls (A, C, E, G, and I). (K–N) Whole-mount immunohistochemistry showing engrailed protein levels (detected by 4D9 antibody) in control MO (K) or ip6k2^SPMO (L) embryos at 24 hpf. Engrailed was not detected in ip6k2^SPMO embryos (L). M and N overlay engrailed immunohistochemistry and TO-PRO3-stained DNA. (O–R) Lateral views of embryos at 24 hpf stained for myosin heavy chain using F59 antibody to visualize slow muscle cells (O and P) and F-actin using rhodamine-phalloidin to reveal both fast and slow muscle cells (Q and R). Dorsal is toward the top and anterior to the left.

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Acknowledgments

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