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Fig. 5 Nocodazole-treated embryos lack both EB3-GFP puncta and axonal movement of EB3-GFP. (A) Kymograph of an axon made from a 10-minute time-lapse recording of a neuron from a DMSO-vehicle-treated embryo, demonstrating predominantly anterograde movement of discrete puncta of EB3-GFP fluorescence (n=16). (B) Kymograph of an axon made from a 10-minute time-lapse recording of a neuron from a nocodazole-treated embryo, demonstrating absence of axonal movement of EB3-GFP fluorescence (n=10). Horizontal scale bar: 20 μm, vertical scale bar: 5 minutes. See supplementary material Movies 7 and 8.
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Full text @ Dis. Model. Mech.