Fig. 4

Fig. 4 Full-length clones of transplanted wild-type Schwann cells can rescue nerve position in sox10/cls^t3 mutants. (A) Wild-type 3 dpf embryo carrying the FoxD3:GFP(17) transgene, labeling the PLLn, motor nerves and some pigment cells. (B) FoxD3:GFP(17) cells have been transplanted into a sox10/cls^t3 mutant host, creating a clone of wild-type Schwann cells that traverse the entire length of the PLLn (3 dpf). (C) Transplanted wild-type Schwann cells can reposition the mutant nerve in the subepidermal space, below the basement membrane (arrowheads). (D) Transplanted wild-type Schwann cells partially rescue the mutant nerve, positioning it within the epidermal basement membrane (arrowheads). Insets show the presence of myelinated axons in C,D. Scale bars: 200 μm for A,B; 2 μm for C,D. Schwann cells are pseudocolored green and axons red in C,D. Abbreviations: ep, epidermis; m, muscle.