IMAGE

Fig. 7

ID
ZDB-IMAGE-100816-7
Genes
Source
Figures for Fett et al., 2010
Image
Figure Caption

Fig. 7 Parkin deficiency has no effect on tyrosine hydroxylase levels in zebrafish.

(A) In situ hybridization using an antisense RNA probe for tyrosine hydroxylase (TH) as a marker for dopaminergic neurons. Staining of parkin knockdown and control-injected zebrafish larvae shows large interindividual variations in both the control and parkin knockdown group. Dorsal views of three-day-old larvae. (B) There is no correlation between the efficiency of parkin knockdown and TH mRNA levels. TH mRNA levels were quantified in parkin-knockdown zebrafish using semi-quantitative real-time PCR. The amount of zebrafish TH-specific mRNA was normalized to β-actin in parkin GT-grip-injected zebrafish larvae compared to control-injected embryos at day 3 after injection.The graph shows the results of 5 independent experiments. In each experiment 10–15 zebrafish larvae per group were analyzed.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One