ZFIN Image: Goody et al., 2010, Fig. 7

Image

Figure Caption

Fig. 7 Nrk2b is cell autonomously required for Paxillin localization to MTJs. (A) Cartoon showing dextran labeled wild-type cells being transplanted into a nrk2b morphant host at blastula stage. (B–D) Side mount, anterior left, 26 hpf nrk2b morphant hosts with transplanted wild-type cells (red), β-Dystroglycan antibody staining (light blue, panel B), pY397 FAK antibody staining (dark blue, panel C), and Paxillin antibody staining (green, panel D). Note that β-Dystroglycan and pY397 FAK robustly localize to MTJs in nrk2b morphants in the presence and the absence of transplanted wild-type cells. Note that robust Paxillin localization to the MTJ in nrk2b morphants is rescued in the transplanted wild-type cells, but not in the Nrk2b-deficient cells surrounding them.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 344(2), Goody, M.F., Kelly, M.W., Lessard, K.N., Khalil, A., and Henry, C.A., Nrk2b-mediated NAD+ production regulates cell adhesion and is required for muscle morphogenesis in vivo: Nrk2b and NAD+ in muscle morphogenesis, 809-826, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.