IMAGE

Fig. 4

ID
ZDB-IMAGE-100603-3
Genes
Source
Figures for Grinblat et al., 2001
Image
Figure Caption

Fig. 4 Early pattern in the ectoderm, marked by zic3 expression, is regulated by bone morphogenetic protein (BMP) signaling. Embryos stained by whole-mount in situ hybridization (ISH) for expression of zic3 (orange in A, purple in C-F) and bmp2b/swr (purple in A). All embryos are shown in animal pole view, dorsal to the right. A: zic3 (orange) and bmp2b/swr (purple) are expressed in complementary domains in wild-type embryos. B: Diagram of a specification map, derived from in vitro explant culture assays, of the early gastrula ectoderm (Grinblat et al., [1998]). V, ventral non-neural ectoderm; A, anterior forebrain; P, posterior forebrain. C: Embryo derived from a din/+x din/+ cross, showing a wild-type pattern of zic3 expression. D: Sibling embryo from the same cross, showing a strong reduction in dorsal zic3 expression. Dorsal marginal staining remained unaffected (not shown). E: Embryo derived from a snh/+ x snh/+ cross, showing a wild-type pattern of zic3 expression. F: Sibling embryo from the same cross, showing up-regulation of zic3 expression in ventral posterior ectoderm (white asterisk). Note that the animal pole is still devoid of zic3 expression. d, dorsal; white asterisk marks ventral side.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.