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Fig. S4 Apico-basal polarity appears normal in pard3 MO-injected embryos. Cross sections through the hindbrain region of mGFP-injected control (A,A′,C,C′) and mGFP, pard3 MO co-injected (B,B′,D,D′) embryos at 12–13 som. Sections from the same embryos were immunolabeled using either anti-ZO-1 (A,A′,B,B′) or anti-γ-tubulin (C,C′,D,D′). White dotted line delineates the neural rod. Double arrowheads indicate length of cell. Brackets show the distribution of γ-tubulin throughout the neuroepithelium. Scale bar: 20 μm.
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Reprinted from Developmental Biology, 341(2), Hong, E., Jayachandran, P., and Brewster, R., The polarity protein Pard3 is required for centrosome positioning during neurulation, 335-345, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.