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Fig. 3 AGN192403 targets the imidazoline receptor. (A) Zebrafish fins were amputated at approximately the level of the first fin ray bifurcation. Fish were treated with 50 μM clonidine, 50 μM Idazoxan, or 1% DMSO (control). Regeneration was allowed to proceed for 72h postamputation (hpa) at 33°C. At 72 hpa, the length of fin ray regeneration (microns) was measured. For all measurements we used the third dorsal fin ray and measured from the original amputation plane to the distal tip of the regenerating fin. Data represent means ± standard error of the mean (SEM). n = 10 for each group. (B) Qualitative RT-polymerase chain reaction for the zebrafish NISCH gene. cDNA was prepared from 72 hpa fin regenerates and used as template to amplify the zebrafish NISCH gene. A 600–700 bp NISCH band is strongly amplified from regenerating caudal fin (arrow, lane 2). No amplification of NISCH is seen in controls lacking primer (lane 3) or cDNA template (lane 4). Lane 1 contains 100 bp ladder.