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Fig. S1 Intraocular Injection of LY294002 in zebrafish larvae. A) ∼5 dechorionated zebrafish larvae (48 hpf in the figure) are immobilized in CyGEL at RT and oriented with left eye facing upwards. Dechorionation is extremely important as non-dechorionated larvae collapse in contact with CyGEL. B) Intraocular injection (0.1–0.2 μl/eye) is performed with a heat pulled glass capillary. C) After intraocular injection the Petri dish is placed on a chilled ice pad to melt CyGEL and larvae are transferred gently but immediately to a plate with RT embryo medium for recovery.
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