ZFIN Image: Gribble et al., 2009, Fig. 3

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Figure Caption

Fig. 3 A single insertion line on chromosome 2 [Tg(-3.5dbx1a:gfp)^zd3] expresses the dbx1a-3.5kb enhancer in an endogenous pattern. A-C: Lateral views of live Tg(-3.5dbx1a:gfp)^zd3 embryos. Boxed region in B is enlarged in inset. D-F: In situ hybridization for gfp mRNA from the same insertion line. Starting at 15 hpf, GFP protein and mRNA are expressed throughout the anterior/posterior extent of the spinal cord. G-I: Cross-sections through the spinal cord of transgenic embryos, stained for gfp mRNA. J: Double staining for dbx1a mRNA (blue) and GFP protein (red). K, L: Staining with 3A10 antibody (red), which marks CoPA interneurons, and GFP (green). Arrows indicate double-labeled CoPA cells. M, N: Embryos treated with cyclopamine from 50% epiboly to 24 hpf show ventral expansion of transgene expression compared with DMSO-treated controls.

Acknowledgments

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