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Fig. S1 Dmrt2b translation is blocked by non-overlapping morpholinos. (A) The Dmrt2b-MO1 and Dmrt2b-MO2 target sequences are shown in relation to the 5′UTR region of the Dmrt2b mRNA sequence. (B) Western blot assay showing Dmrt2b translation in the embryos injected with Cont-MO, Dmrt2b-MO1, Dmrt2b-MO2 and Dmrt2b-MO (Dmrt2b-MO1+Dmrt2b-MO2). The signal of Dmrt2b protein were significant reduced in Dmrt2b-MO1, Dmrt2b-MO2 and Dmrt2b-MO injected embryos.
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