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Fig. 11 Zebrafish embryos display vascular collapse and then recover over a 24 h time period at low PlcHR doses.
Transgenic zebrafish embryos expressing EGFP in endothelial cells and RFP in erythrocytes (Tg(fli1:EGFP):Tg(gata1:dsRED)) [41],[42]) were injected with 1 ng of PlcHR, or its catalytic mutant, as before. (A–F) Single and double prime panels denote visualization of EGFP-labeled endothelial cells or RFP-labeled blood cells in the same embryos, respectively. At 6 hpi, embryos injected with PlcHR displayed little or no circulation (compare D″ with A″, B″). (E–F) Circulation was partially restored by 12 hpi, and embryos recovered by 24 hpi. Representative pictures of embryos from one of three independent experiments; n>20 per condition. White arrow denotes blood flow in dorsal aorta. Scale bar indicates 250 μm.