ZFIN Image: Mei et al., 2009, Fig. 3

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Figure Caption

Fig. 3 Phosphatase activity of zebrafish Mtmr8.

Different extracts from Cont MO embryos, Mtmr8 morphants and Mtmr8 morphants co-injected with Mtmr8 mRNA were prepared at 24 hpf (30 embryos/group) and subjected to Western blot detection with anti-Akt antibody or anti-phospho-Akt (pAkt) antibody. The corresponding histograms (right panels) plot the relative Ser(P)473-Akt to total Akt ratio (Relative p-Akt/Akt ratio), determined by band intensities that were analyzed by Scion software, with the ratio in experiment extracts normalized to the ratio determined in Cont MO injected embryo extracts. Results represent mean±SD of three separate experiments. *P<0.05.

Acknowledgments

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