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Fig. 6 BrdU mitotic pileup in clones injected with 50 pg egfp mRNA (A–C) or 50 pg 6MT-cdc25a mRNA (D–F). Embryos were injected with mRNA mixed with 70 K rhodamine dextran (C, F) then exposed to BrdU and nocodazole at 28 °C for 15 min. Embryos were then stained with anti-BrdU monoclonal antibody (white in B, E; green, C, F) and nuclei were counterstained with TO-PRO 3 (white in A, D; blue in C, F). Red arrowheads in panels A, B, D and E indicate BrdU-positive mitotic figures, and white arrowheads in A indicate BrdU-negative mitotic figures.
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Reprinted from Developmental Biology, 326(1), Dalle Nogare, D.E., Pauerstein, P.T., and Lane, M.E., G2 acquisition by transcription-independent mechanism at the zebrafish midblastula transition, 131-142, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.