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Fig. 1 The Early Granuloma Enhances Bacterial Replication by RD1-Dependent Recruitment and Infection of New Cells
(A and B) Fluorescent images of WT (A) and ΔRD1 (B) infected lesions at 48, 72, and 96 hr post-infection (hpi). Scale bars, 10 μm.
(C and D) Three-dimensional reconstructions of granulomas in WT (C) and ΔRD1 (D) infection, showing Hoechst-positive nuclei rendered white (indicated by white arrows in D) accumulated over 24 hr. Scale grid, 5 μm per square.
(E) Comparison of numbers of Hoechst-positive arrivals in WT and ΔRD1 lesions after 24 hr. Brackets indicate granulomas in the same embryo. Horizontal lines represent means. p value from Mann-Whitney test.
(F–H) Neutral red-stained optic tectum macrophages at 5 days post-hindbrain injection with WT Mm (F), ΔRD1 Mm (G), or no infection (H). Macrophages detected using neutral red dye (upper panels) and their outlines overlayed on images of the fluorescent bacteria present (lower panels—no fluorescent image collected for uninfected embryos). Scale bars, 100 μm.
(I) Numbers of optic tectum macrophages at 2 dpi and 5 dpi. p = 0.47 (2 dpi) and 0.01 (5 dpi) (Kruskal-Wallis test, p < 0.05).
Reprinted from Cell, 136(1), Davis, J.M., and Ramakrishnan, L., The role of the granuloma in expansion and dissemination of early tuberculous infection, 37-49, Copyright (2009) with permission from Elsevier. Full text @ Cell