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Figure Caption
Fig. 8 Apoptosis of DTA-expressing embryos. In situ TUNEL analysis was done by using central, transverse frozen sections of zebrafish embryonic eyes. DTA-expressing or control embryos at 36 and 54 hpf were embedded in OCT compound and frozen sectioned. FITC conjugate–dUTP-labeled cells were visualized with anti-FITC-horseradish peroxidase conjugate antibody and DAB. Samples were examined under Normarsky microscopy (Axioplan; Carl Zeiss). Scale bars, 50 μm.
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Reprinted from Developmental Biology, 255(1), Kurita, R., Sagara, H., Aoki, Y., Link, B.A., Arai, K.-I., and Watanabe, S., Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish, 113-127, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.