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Fig. 1 Lens-specific expression of zebrafish αA-crystallin mRNA. Whole-mount in situ of zebrafish embryo (29 hpf) was done by using DIG-labeled fragment of zebrafish αA-crystallin (left panel). Hybridization with control sense probe resulted in no signal (data not shown). In situ hybridization of frozen sectioned zebrafish embryos (right panel). Embryos were fixed by PFA and frozen in OCT. Sections were 10 μM thick, and hybridized with DIG labeled fragments. Scale, 50 μM.
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Reprinted from Developmental Biology, 255(1), Kurita, R., Sagara, H., Aoki, Y., Link, B.A., Arai, K.-I., and Watanabe, S., Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish, 113-127, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.