ZFIN Image: Sharma et al., 2008, Fig. 10

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Figure Caption

Fig. 10 Localization of activated Src-family PTKs in the zebrafish oocyte. Zebrafish oocytes were fixed at 1.5 mpi to capture the fertilization cone with attached sperm. They were then processed for immunofluorescence in the presence of phosphatase inhibitors and then incubated with the clone-28 antibody to label activated (dephosphorylated) Src-family PTKs (0.5 μg/ml). Bound antibody was detected with goat anti-mouse IgG conjugated to Alexa fluor-488. The fertilizing sperm is visible as a round spot at the apex of the fertilization cone which protrudes from the right hand edge of the oocyte. Src-family PTKs were strongly activated in the region of the fertilization cone indicating the response of the oocyte to fertilization. Magnification is indicated by the bar which represents 25μm.

Acknowledgments

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