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Fig. S1 Effect of DEAB and SU5402 treatment on neural plate size. Incubation of embryos in 10 μM DEAB from shield stage until 8.5 hpf results in reduction of mdkb expression (A,B; marked by arrows), while expression of tfap2 in non-neural ectoderm and neural plate size is not affected (E,F). Incubation of embryos with SU5402 results in a reduction of both, mdkb expression (C,D) and neural plate size as indicated by an expanded tfap2 expression domain (G,H). erm expression is reduced in SU5402 treated embryos (I, J, arrowhead), as described in the literature (Raible and Brand, 2001). (K,L) Phenotypes of live embryos at 24hpf treated with 50μM SU5402, note severe necrosis.