Fig. 4 The percentage of maximal respiration that is used basally is lowest in pre-midblastula transition (MBT) embryos. A: The rate of maximal oxygen consumption was measured in embryos at different stages by treating embryos with the mitochondrial uncoupler CCCP (squares, left y-axis). The percentage of maximal oxygen consumption that is consumed basally was calculated for each sample and averaged (triangles, right y-axis). All error bars are ±SD. B,C: Live embryos at 16 cells (B) or sphere-stage (C) were stained with MitoTracker dye (red) and Hoescht (blue) and imaged by fluorescent microscopy. Images are representative of five independent repetitions. Scale bar = 100 μm.
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