ZFIN Image: Li et al., 2000, Fig. 11

Image

Figure Caption

Fig. 11 Absence of Pax6.1 expression results in severe abnormalities of head development. (A) The 48-h embryos injected with double-stranded control RNA had normal phenotypes. Microinjection of double-stranded Zf-Pax6.1 RNA into single-cell zebrafish embryos resulted in 25% of the embryos having reduced eyes often accompanied by diminished and disorganized forebrains. (B) In situ hybridizations demonstrated that 24-h embryos lack Zf-Pax6.1 expression following double-stranded Zf-Pax6.1 RNA injection into single-cell embryos, although double-stranded control RNA injection had no apparent effect on Zf-pax6.1 expression. Scale bars = 100 μm.

Acknowledgments

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Reprinted from Developmental Biology, 217(2), Li, Y.X., Farrell, M.J., Liu, R., Mohanty, N., and Kirby, M.L., Double-stranded RNA injection produces null phenotypes in zebrafish, 394-405, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.