ZFIN Image: Zhu et al., 2003, Fig. 8

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Figure Caption

Fig. 8 Partial purification of ovarian progestin receptor and monoclonal antibody screening. (a) Elution profile of solubilized seatrout ovarian membrane extracts on a DEAE column showing peaks of UV absorbance and progestin (17,20β,21-trihydroxy-4-pregnen-3-one) binding activity. Each peak was pooled and concentrated with a 10-kDa molecular mass cutoff filter. Specific progestin binding of each pooled fraction is indicated near each peak. (b) Silver stain of SDS/PAGE gel of the three pooled fractions. Numbers refer to the pooled fractions obtained from DEAE chromatography. (c) Western blot of solubilized ovarian membrane proteins using monoclonal antibody PR10-1. I, solubilized seatrout ovarian plasma membrane proteins; II, seatrout ovarian cytosolic proteins; III, partially purified membrane proteins in fraction 1.

Acknowledgments

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