Fig. 5

Fig. 5 zOtx1-induced cell aggregates are compatible with the formation of anterior brain regions. To restrict the cell aggregates to a smaller region of the gastrulating embryo, embryos were injected with zOtx1-wt RNA plus GFP RNA into a single nonmarginal, noncentral cell at the 32-cell stage. A single embryo is shown here at 9 (A, B), 12 (C, D), and 24 (E, F) hpf in lateral (A, C, E) and dorsal (B, D, F) views. Descendants of the injected cell in this embryo remain within the prospective anterior brain at 9 hpf, in the developing head region at 12 hpf, and mostly become part of the diencephalon and neural retina at 24 hpf. Arrowheads point to cell aggregates, and for the 24-hpf embryos, to groups of cells located in the forebrain, midbrain, and neural retina. Scale bar, 100 μm (all at same magnification).