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Fig. 5 Transgene signal is RA inducible and dependent. (A) Treatment of embryos with 1 μM RA at 18 hpf induces transgene expression throughout the neural tube and neural retina by 24 hpf (+RA, left) compared to an ethanol-treated control (-RA, right). (B) Treatment of embryos with 10 μM DEAB, an RA synthase inhibitor, at 18 hpf significantly reduces transgene signal in the neural tube and abolishes it in all other regions of expression by 24 hpf (+DEAB, right) compared to DMSO-treated control (-DEAB, left). (C) Expression in the anterior nervous system responds to RA induction at varying levels when treated with 0.1 μM RA at 18 hpf. (D) Details of posterior induction, including the pronephric ducts, immature notochord cells, neural tube, and enveloping layer when embryos are treated at 1 μM RA at 18 hpf. Bars, 100 μm. Scale for A is same as in B. Scale for C is same as in D.
Reprinted from Developmental Biology, 229(1), Perz-Edwards, A., Hardison, N.L., and Linney, E., Retinoic acid-mediated gene expression in transgenic reporter zebrafish, 89-101, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.