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Fig. S1 Expression of hypothalamic genes relative to DA neurons. In situ hybrydization with RNA probes directed against fezl (a,b), otpb (c), pacab1 (e,f,h) and pac1 (g) followed by immunostaining with an anti-tyrosine hydroxylase (TH) antibody. (D) Sagittal paraffin sections (6 μM) of an embryo which was subjected to in situ hybridization with an otpb- directed probe followed by anti-TH immunohistochemistry. HB, hindbrain; hpf, hours post fertilization; Hyp, hypothalamus; MB, midbrain; NPO, neurosecretory preoptic area; PT, posterior tuberculum; Tel, telencephalon.
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