IMAGE

Fig. 5

ID
ZDB-IMAGE-080325-98
Source
Figures for Lachnit et al., 2008
Image
Figure Caption

Fig. 5 Enhanced lamellipodia formation of EVL cells. Animal pole confocal z-projections of Alexa488-Phalloidin stained embryos during epiboly. (A–F) EVL cells. (A, C, E) WT EVL cells with few detectable lamellipodia. (B, D, F) MZspgm793 EVL cells with enhanced lamellipodia formation at the cell–cell contact site of EVL cells, projecting toward the deep cells, (A, B) at 60% epiboly, (C, D) at 75% epiboly, and (E, F) at 90% epiboly. Images show z-projections of confocal stacks of images using the depth coding method, to visualize the localization of lamellipodia. Depth scale bar is depicted beneath panels E and F. Scale bar: 20 μm. (G) Measurements of the lamellipodia area including SE: 60% epiboly: WT = 100 ± 0%, MZspgm793 = 100.75 ± 0% (no lamellipodia detectable). 75% epiboly: WT = 100 ± 40%; MZspgm793 = 1072 ± 67% (p = 3.157 · 10- 08). 90% epiboly: WT = 100 ± 20%; MZspgm793 = 2804 ± 290% (p = 2.563 · 10- 06). Measurements were performed with Volocity software. Data were normalized to WT results which was set as 100%. Asterisks highlight significant differences. Number of analyzed embryos is depicted within or above bars.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.