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Fig. 4

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ZDB-IMAGE-080325-91
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Figures for Lachnit et al., 2008
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Fig. 4 Delay in cell shape changes of marginal EVL cells. (A–F) Confocal z-projections of Alexa488-Phalloidin stained embryos showing a delay in flattening of marginal EVL cells. (A, C, E) WT; (B, D, F) MZspgm793 embryos. (A, B) 50% epiboly; (C, D) 75% epiboly; (E, F) 90% epiboly. All MZspgm793 stages are time-matched to wild-type embryos. yc: yolk cell. Scale bar: 100 μm. (G) Measurements of the average border cell length of 10–15 marginal EVL cells including SE. 30% epiboly: WT = 100 ± 8%; MZspgm793 = 103 ± 6% (p = 0.205). 50% epiboly: WT = 100 ± 5%; MZspgm793 = 115 ± 4% (p = 0.029). Shield stage: WT = 100 ± 4%; MZspgm793 = 124 ± 7% (p = 0.011). 75% epiboly: WT = 100 ± 2%; MZspgm793 = 246 ± 2% (p = 0.069). 90% epiboly: WT = 100 ± 12%; MZspgm793 = 117 ± 5 (p = 0.122). Data were normalized on WT results set as 100%. Asterisks highlight significant differences. (H) Measurements of the length to width ratio of marginal EVL cells. For each stage the length to width ratio of 10 adjacent cells was determined in 10 embryos of each wild-type or MZspgm793. The graph represents average data from 100 cells for each genotype and stage, bars indicate standard deviation. 50% epiboly: MZspgm793 = 1.24 ± 0.42; WT = 1.26 &p00 cells for each genotype and stage, bars indicate standard deviation. 50% epiboly: MZspgp = 0.8004; paired Student′s t-Test); 75% epiboly: MZspgm793: 1.34 ± 0.53; WT = 1.63 ± 0.90 (p = 0.2349); 90% epiboly: MZspgm793 = 1.81 ± 0.72; WT = 2.93 ± 1.25 (p 

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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.