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Fig. 2 Epiboly delay of the DEL and EVL. (A–D) Confocal z-projections of Sytox green stained embryos at 85% epiboly stage. MZspgm739 embryos show a strong DEL (arrows) and EVL (arrowheads) retardation. (A, B) Lateral and (C, D) vegetal views. (E) Measurements of the mean diameter “d” of diametral DEL margins: WT = 251 ± 26 μm, MZspgm793 = 605 ± 6 µm, p = 2 · 10- 08. (F) Measurements of the mean diameter “d” of diametral EVL margins: WT = 128 ± 31 μm, MZspgm739 = 429 ± 13 µm (p = 1 · 10- 06). Scale bar: 100 μm.
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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.