IMAGE

Fig. S2

ID
ZDB-IMAGE-080325-74
Genes
Source
Figures for Bessa et al., 2008
Image
Figure Caption

Fig. S2 meis1-MO injection reduces rhombomeres-3 krox20 expression specifically. (A) Ctrl-MO has no effect on krox20 expression in rhombomeres 3 (r3) or 5 (r5). (B) meis1-MO embryos show a specific reduction of r3 krox20 expression (red arrowhead). (C) Injection of a meis1, 2.2, 3 and 4 MO cocktail results in an even stronger inhibition of r3 krox20 expression (red arrowheads). (D) This effect is partially rescued by injection of GFP-meis1 mRNA (meis1GFP) (black arrows). GFP-meis1 is insensitive to meis1-MO, since the N-terminal tagging of meis1 with GFP destroys the meis1-MO target sequence in the GFP-meis1 mRNA. These results phenocopy the reported effect on r3 krox20 expression caused by injection of a dominant-negative Meis form (Waskiewicz et al., 2001) and thus confirm the biological specificity of the Meis mopholinos used in this study.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development