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Fig. S6 Maternal contribution to the YCL phenotype. (A, B) Alexa488-Phalloidin staining. (C–E) Anti-β-Tubulin antibody immunohistochemistry with secondary antibody/HRP/DAB detection. (A–C) 75% epiboly, (D, E) 50% epiboly embryos. (A, D) WT; (B, C, E) Mspgm793. (E) Microtubule track formation in Mspgm793 embryos is slightly distorted, but microtubule free patches are not observed. Scale bars: 100 μm.
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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.