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Fig. 3 RA Signaling Is Required for Proximal Segment Fates and to Prevent the Expansion of Distal Segment Fates. (A) Expression of segment markers and mhc at 48 hpf in wild-type embryos and raldh2 morphants, and wild-type embryos that were incubated with either DMSO (control) or DEAB for indicated times during development, by double whole-mount in situ hybridization. Brackets and arrows indicate expression domains. Abbreviations are: %e (percent epiboly), s (somite stage). Dorsal views are shown, with anterior to the left, with the exception of lateral views for gata3 and aqp3 expression. (B) Summary of nephron segmentation with respect to embryo somite number for each DEAB treatment.

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