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Figure Caption
Fig. 4 Alk4 S275M restores phospho-Smad2 signaling to SB-431542-treated animal caps and embryos. Embryos (Xenopus and zebrafish) were injected with 100 pg Alk4 S275M or 100 pg Alk4 WT. Western blots for phospho-Smad2 were performed on (A) stage 8.5 Xenopus animal caps treated with 100 μM SB-431542 or DMSO followed by 2 nM soluble activin protein, and (B) 50% epiboly zebrafish embryos treated with 100 μM SB-431542 at 16-cell stage. Cytoskeletal actin and tubulin were used as loading controls for panels A and B, respectively.
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Reprinted from Developmental Biology, 295(2), Ho, D.M., Chan, J., Bayliss, P., and Whitman, M., Inhibitor-resistant type I receptors reveal specific requirements for TGF-beta signaling in vivo, 730-742, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.