ZFIN Image: Pollard et al., 2006, Fig. 4

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Figure Caption

Fig. 4 Expression and morpholino antisense knockdown of lama5 in zebrafish embryos. Whole-mount in situ hybridisation of lama5 mRNA in zebrafish embryos at 64-cell stage (A), tailbud stage (B, dorsal) and 20-somite stage (C, lateral). Injection of antisense MO directed against lama5 mRNA results in CNS disruptions and defective extension of the yolk sac by 28 hpf (E) compared to control MO injected (D). Notochord, however, appears fully differentiated, by morphology and laminin-1 immunoreactivity (H) compared to control MO-injected notochord (G). Embryos injected with lama5 MO display strong persistent expression of the early chordamesoderm marker, ehh (K), whose expression is extinguished by this stage in control MO-injected embryos (J). Injection of lama5 MO into bal mutant embryos results in severe disruption of many tissues (F), though laminin-1 immunoreactivity is retained (I).

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Acknowledgments

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Reprinted from Developmental Biology, 289(1), Pollard, S.M., Parsons, M.J., Kamei, M., Kettleborough, R.N., Thomas, K.A., Pham, V.N., Bae, M.K., Scott, A., Weinstein, B.M., and Stemple, D.L., Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation, 64-76, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.