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Fig. S1 Efficient Differentiation of Rostral Cardiomyocytes in clo Mutant Embryos Requires Hand2 (A-L) Two-color fluorescent in situ hybridization at the 20 somite stage, dorsal views, anterior to the top. Scale bar represents 100 μm. (A-D) Overlays of cmlc2 (E-H) and nkx2.5 (I-L) expression. Wild-type embryos (A,E,I) exhibit efficient myocardial differentiation of nkx2.5- expressing cells, in contrast to the limited differentiation observed in han mutants (B,F,J). Examination of han;clo double mutants (D,H,L) demonstrates that ectopic myocardial differentiation in nkx2.5-expressing rostral ALPM (arrows, C,D,G,K,L), as observed in clo mutants (C,G,K), also requires Hand2 function. Therefore, cmlc2 expression in han;clo double mutants (H) appears similar to cmlc2 expression in han mutants (F).
Reprinted from Developmental Cell, 13(2), Schoenebeck, J.J., Keegan, B.R., and Yelon, D., Vessel and blood specification override cardiac potential in anterior mesoderm, 254-267, Copyright (2007) with permission from Elsevier. Full text @ Dev. Cell