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Fig. S3 Heat-induced rapid and generalized expression of phsp70:cdx4 transgene. Expression of cdx4 (purple) and myod (red in adaxial cells) in wild-type embryos (A) and their siblings carrying one copy of a phsp70:cdx4 transgene (B) that were heat shocked for 1 hour at 37°C starting at the three-somite stage (11 hpf) and immediately fixed. From three crosses between a wild-type and a transgenic heterozygous fish, 48% of embryos showed ubiquitous cdx4 expression (n=428, B). Upon genotyping, 100% of embryos expressing cdx4 ubiquitously were also positive for the transgene and 100% of embryos with a normal cdx4 pattern of expression tested negative for the transgene (n=20 for each class). Embryos are shown in dorsal view, anterior to the left. Scale bars: 100 μm.