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Fig. 4

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ZDB-IMAGE-070421-3
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Figures for Shankaran et al., 2007
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Fig. 4 Expression pattern analysis of the mHes5 homologues in the rostral PSM. (A–C, F) wt expression pattern of her15. At 8- to 10-somite stage her15 expression was observed in one to two thin stripes in the rostral half PSM (A–C). These stripes occur in a double segmental distance at the future posterior somite borders of S1 and S-1, or when compared to myoD are co-expressed with the last myoD stripe and the myoD stripe within the last forming or formed somite (F). (D, E) her15 expression in fss. (G–I) wt expression pattern of her12 (blue) double-stained with myoD (red). (J, K) her12 expression in fss. (L–N) wt expression pattern of her4.1. In contrast to her15, the stripes of her12 and her4.1 expression occur in a single segmental distance in the rostral PSM (G–I and L–N, respectively). (F) wt expression of her15 (in blue) compared to myoD (in red). Black arrowhead points to the anterior-most her15 and her4 expression stripe, respectively, at the forming segment border. White arrowhead marks the posterior her15 stripe, which is located in the last myoD stripe. Black arrow indicates the position of the two her12 stripes matching with the penultimate and ultimate myoD stripe, respectively. White arrow marks the last formed segment border. (O) Schematic representation of the expression stripes of the mHes5 homologues in the rostral PSM. Location of the striped expression in the rostral PSM is compared between her4.1, her12 and her15. Note, cyclic expression in the posterior is indicated by roman numbers I and II below the respective drawing. S0 = somite, which will be formed next; S-1 = prospective future somite posterior to S0.

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Reprinted from Developmental Biology, 304(2), Shankaran, S.S., Sieger, D., Schroter, C., Czepe, C., Pauly, M.C., Laplante, M.A., Becker, T.S., Oates, A.C., and Gajewski, M., Completing the set of h/E(spl) cyclic genes in zebrafish: her12 and her15 reveal novel modes of expression and contribute to the segmentation clock, 615-632, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.