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Fig. 5 A,B: Expression of wt1 in adult tissues. Indicated organs were isolated from 6 month old zebrafish (A) and mice (B). Wt1 expression levels were determined by RT-PCR. Fragment sizes for zebrafish wt1 were identical to those in Figure 3. In case of the mouse tissues, the wt1 amplicon comprised 194 bp and the β-actin amplicon 165 bp. To verify the absence of genomic DNA contamination, an aliquot of the testis RNA sample from zebrafish and mouse was used for polymerase chain reaction without reverse transcription (-rt).
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