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Fig. 7 Tenascin-C morpholinos induce aberrant branching of ventral motor nerves. For orientation, see Figure 1A. A-C: At 24 hours postfertilization (hpf), tenascin-C morpholino 1 (B) and tenascin-C morpholino 2 (C), but not control morpholino (A), abolish tenascin-C immunoreactivity in the region of the horizontal myoseptum (arrows) and strongly reduce it in vertical myosepta (arrowhead). D-J: At 33 hpf, aberrant branches of anti-tubulin-labeled ventral motor nerves were observed in embryos injected with tenascin-C morpholino 1 (arrows in F-H) or injected with tenascin-C morpholino 2 (arrows in I,J), but not in embryos injected with control morpholino (E) or in uninjected embryos (D). D,F: Arrowheads indicate the level of the horizontal myoseptum for D,E and F-J, respectively. Scale bar = 50 μm in J (applies to A-J).