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Fig. 5

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ZDB-IMAGE-050823-3
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Figures for Nowak et al., 2005
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Fig. 5 During normal zebrafish development, perp is expressed independently of p53 and the related proteins ΔNp63 and TAp73. (a,b,d-f) show whole-mount in situ hybridizations at 36 hpf; (a) p53 expression, which is most prominent in the brain, eyes, and gut. (b) p63 expression, most prominent in the skin. (c-e) perp expression in uninjected control embryo (c), and in p53 morphant (d), and ΔNp63 morphant embryo (e). perp expression in eyes, midbrain-hindbrain boundary region (mhb), and gut (gt) of p53 morphant, and in the skin (epi) of ΔNp63 morphant are indicated. (f) Comparative semiquantitative RT-PCR analysis of perp transcript levels in p53 morphants (lane 2), ΔNp63 morphants (lane 3), TAp73 morphants (lane 4), p53/TAp73 double-morphants (lane 5) and p53/Tap73/ΔNp63 triple-morphants (lane 6) at 48 hpf, with ef1α cDNA amplification as control. Efficiency of used p53 MO was confirmed by parallel coinjections with mdm2 MO, leading to a rescue of the mdm2 morphant phenotype, as described elsewhere.20 Efficiency of ΔNp63 and TAp73 MOs was confirmed by the skin and craniofacial phenotypes of injected sibling embryos at later stages of development23, 26

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ZFIN also wishes to thank Matthias Hammerschmidt for permission to reproduce figures from this article.
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