Gene Trap Construct

Gt(LOX71-FRT-GAL4-VP16-FRT-LOX66,Xla.Crygc:BFP)

ID

ZDB-GTCONSTRCT-181024-1

Previous Names

Switchblade (1)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

BFP (1)
FRT (1)
GAL4 (1)
LOX66 (1)
LOX71 (1)
VP16 (1)

[Em λ][Ex λ]

Fpbase:BFP

Contains Sequences

None

Note

This is a On/Off/On gene trap cassette. It can be turned off with Flp recombinase which will invert the the gene trap cassette. To flip the cassette back on treat with Cre recombinase.

Plasmid Map

None

Genomic Features

That Utilize Gt(LOX71-FRT-GAL4-VP16-FRT-LOX66,Xla.Crygc:BFP) Construct

Genomic Feature	Affected Genomic Regions
tpl53Gt	crip2l
tpl54Gt	dnah5
tpl55Gt	lztfl1
tpl57Gt	ankrd12
tpl58Gt	tbx5a
tpl59Gt	sypb
tpl95Gt	fli1rs
tpl96Gt	rbpms2b

Transgenics

That Utilize Gt(LOX71-FRT-GAL4-VP16-FRT-LOX66,Xla.Crygc:BFP) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
ankrd12^{tpl57Gt/tpl57Gt}	ankrd12		Fig. 1 from Grajevskaja et al., 2018
crip2l^{tpl53Gt/tpl53Gt}	crip2l		Fig. 1 from Grajevskaja et al., 2018
sypb^{tpl59Gt/tpl59Gt}	sypb		Fig. 1 from Grajevskaja et al., 2018
tbx5a^tpl58Gt/+	tbx5a	Fig. 2 from Grajevskaja et al., 2018
tbx5a^tpl58Gt/+; tpl2Tg/+; tpl48Tg/+	tbx5a	Fig. 4 from Grajevskaja et al., 2018
tbx5a^tpl58Gt/+; tpl2Tg; tpl48Tg	tbx5a	Fig. 3 from Grajevskaja et al., 2018	Fig. 3 from Grajevskaja et al., 2018
tbx5a^{tpl58Gt/tpl58Gt}	tbx5a	Fig. 2 from Grajevskaja et al., 2018
tbx5a^{tpl58Gt/tpl58Gt}; tpl2Tg/+; tpl48Tg/+	tbx5a	Fig. 4 from Grajevskaja et al., 2018

Sequence Information

Other Construct Pages

None

Citations