Gene
myo7aa
- ID
- ZDB-GENE-020709-1
- Name
- myosin VIIAa
- Symbol
- myo7aa Nomenclature History
- Previous Names
-
- myo7a
- mar
- mariner
- Type
- protein_coding_gene
- Location
- Chr: 18 Mapping Details/Browsers
- Description
- Predicted to enable actin filament binding activity and microfilament motor activity. Acts upstream of or within several processes, including auditory behavior; neuron differentiation; and regulation of Rho protein signal transduction. Predicted to be located in cytoskeleton and plasma membrane bounded cell projection. Predicted to be part of myosin complex. Predicted to be active in several cellular components, including actin cytoskeleton; cytoplasm; and microvillus. Is expressed in brain; otic vesicle; sensory system; and tether cell. Used to study Usher syndrome type 1. Human ortholog(s) of this gene implicated in Leber congenital amaurosis; Usher syndrome (multiple); auditory system disease (multiple); and congenital nystagmus. Orthologous to human MYO7A (myosin VIIA).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 10 figures from 7 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- 21 figures from 8 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| hn13 | Allele with one delins | Exon 6 | Unknown | CRISPR | |
| hn14 | Allele with one delins | Unknown | Unknown | CRISPR | |
| ihb221 | Allele with one insertion | Unknown | Unknown | CRISPR | |
| ihb222 | Allele with one delins | Unknown | Unknown | CRISPR | |
| sa11378 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa16521 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa23224 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa28973 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa28974 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa36565 | Allele with one point mutation | Unknown | Splice Site | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-myo7aa | Varshney et al., 2015 | |
| CRISPR2-myo7aa | (3) | |
| CRISPR3-myo7aa | (3) | |
| CRISPR4-myo7aa | Qin et al., 2024 | |
| CRISPR5-myo7aa | Xie et al., 2024 | |
| CRISPR6-myo7aa | Xie et al., 2024 |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| autosomal dominant nonsyndromic deafness 11 | Alliance | Deafness, autosomal dominant 11 | 601317 |
| autosomal recessive nonsyndromic deafness 2 | Alliance | Deafness, autosomal recessive 2 | 600060 |
| Usher syndrome type 1 | Alliance | Usher syndrome, type 1B | 276900 |
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| Human Disease | Fish | Conditions | Citations |
|---|---|---|---|
| Usher syndrome type 1 | myo7aatc320b/tc320b | standard conditions | Riley et al., 2023 |
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Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Band 4.1 domain | Class VII myosin, motor domain | FERM/acyl-CoA-binding protein superfamily | FERM central domain | FERM domain | FERM superfamily, second domain | IQ motif, EF-hand binding site | IRS-type PTB domain | Kinesin motor domain superfamily | Myosin head, motor domain-like | Myosin VII, FERM domain C-lobe, repeat 1 | Myosin VII, FERM domain C-lobe, repeat 2 | MyTH4 domain | MyTH4 domain superfamily | PH-like domain superfamily | P-loop containing nucleoside triphosphate hydrolase | SH3 domain | SH3-like domain superfamily | Ubiquitin-like domain superfamily | Unconventional Myosin ATPase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A0A8M9P9W0 | InterPro | 2185 | ||||||||||||||||||||
| UniProtKB:Q9DGG9 | InterPro | 2179 | ||||||||||||||||||||
| UniProtKB:A0AB32T794 | InterPro | 2232 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
myo7aa-201
(1)
|
Ensembl | 6,781 nt | ||
| mRNA |
myo7aa-202
(1)
|
Ensembl | 7,561 nt | ||
| mRNA |
myo7aa-203
(1)
|
Ensembl | 6,651 nt |
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Interactions and Pathways
No data available
| Name | Type | Antigen Genes | Isotype | Host Organism | Assay | Source | Citations |
|---|---|---|---|---|---|---|---|
| ab1-myo7aa | polyclonal | Rabbit |
|
1 |
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Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg2(myo7aa-gata2a:EGFP) |
|
| 1 | Ernest et al., 2015 | |
| Tg3(myo7aa-gata2a:EGFP) |
|
| 1 | Ernest et al., 2015 | |
| Tg4(myo7aa-gata2a:EGFP) |
|
| 1 | Ernest et al., 2015 | |
| Tg5(myo7aa-gata2a:EGFP) |
|
| 1 | Ernest et al., 2015 | |
| Tg6(myo7aa-gata2a:EGFP) |
|
| 1 | Ernest et al., 2015 | |
| Tg7(myo7aa-gata2a:EGFP) |
|
| 1 | Ernest et al., 2015 | |
| Tg(myo7aa-gata2a:EGFP) |
|
| 1 | Ernest et al., 2015 | |
| Tg(myo7aa-gata2a:mCherry) |
|
| 1 | Ernest et al., 2015 | |
| Tg(myo7aa-gata2a:mCherry-myo7aa) |
|
|
| 1 | Ernest et al., 2015 |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-135H6 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:195128 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_152983 (1) | 7590 nt | ||
| Genomic | GenBank:CU459145 (1) | 191629 nt | ||
| Polypeptide | UniProtKB:A0AB32T794 (1) | 2232 aa |
- Patterson, A.S., Dugdale, J., Koleilat, A., Krauss, A., Hernandez-Herrera, G.A., Wallace, J.G., Petree, C., Varshney, G.K., Schimmenti, L.A. (2024) Vital Dye Uptake of YO-PRO-1 and DASPEI Depends Upon Mechanoelectrical Transduction Function in Zebrafish Hair Cells. Journal of the Association for Research in Otolaryngology : JARO. 25(6):531-543
- Qin, W., Liang, F., Lin, S.J., Petree, C., Huang, K., Zhang, Y., Li, L., Varshney, P., Mourrain, P., Liu, Y., Varshney, G.K. (2024) ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish. Nature communications. 15:56135613
- Smaili, W., Pezet, C., Marlin, S., Ernest, S. (2024) R391 human dominant mutation does not affect TubB4b localization and sensory hair cells structure in zebrafish inner ear and lateral line. Developmental Biology. 517:301-316
- Xie, B., Liang, J., Jiang, J., Zeng, T., Liu, L., Xie, D., Zhu, G., Xiong, L., Zhang, K., Liu, D., Gong, J., Chen, X., Lai, R., Xie, H. (2024) Zebrafish myo7aa affects congenital hearing by regulating Rho-GTPase signaling. Frontiers in molecular neuroscience. 17:14051091405109
- Ezhkova, D., Schwarzer, S., Spieß, S., Geffarth, M., Machate, A., Zöller, D., Stucke, J., Alexopoulou, D., Lesche, M., Dahl, A., Hans, S. (2023) Transcriptome analysis reveals an Atoh1b-dependent gene set downstream of Dlx3b/4b during early inner ear development in zebrafish. Biology Open. 12(6):
- Riley, K.C., Koleilat, A., Dugdale, J.A., Cooper, S.A., Christensen, T.A., Schimmenti, L.A. (2023) Three-Dimensional Structure of Inner Ear Hair Cell Ribbon Synapses in a Zebrafish Model of Usher Syndrome Type 1B. Zebrafish. 20:475447-54
- Shi, T., Beaulieu, M.O., Saunders, L.M., Fabian, P., Trapnell, C., Segil, N., Crump, J.G., Raible, D.W. (2023) Single-cell transcriptomic profiling of the zebrafish inner ear reveals molecularly distinct hair cell and supporting cell subtypes. eLIFE. 12:
- Erickson, T., Pacentine, I.V., Venuto, A., Clemens, R., Nicolson, T. (2020) The lhfpl5 Ohnologs lhfpl5a and lhfpl5b Are Required for Mechanotransduction in Distinct Populations of Sensory Hair Cells in Zebrafish. Frontiers in molecular neuroscience. 12:320
- Koleilat, A., Dugdale, J.A., Christenson, T.A., Bellah, J.L., Lambert, A.M., Masino, M.A., Ekker, S.C., Schimmenti, L.A. (2020) L-type voltage-gated calcium channel agonists mitigate hearing loss and modify ribbon synapse morphology in the zebrafish model of Usher syndrome type 1. Disease models & mechanisms. 13(11):
- Zhang, Y., Wang, Y., Yao, X., Wang, C., Chen, F., Liu, D., Shao, M., Xu, Z. (2020) Rbm24a Is Necessary for Hair Cell Development Through Regulating mRNA Stability in Zebrafish. Frontiers in cell and developmental biology. 8:604026
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