FIGURE

Fig. 6

ID

ZDB-FIG-260714-9

Publication

Zhang et al., 2026 - Mutation of Tubgcp6 induces hematopoietic stem and progenitor cell exhaustion in zebrafish

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Fig. 6

tubgcp3, tubgcp4, and tubgcp5 mutants exhibit similar HSPC deficiency phenotypes.

Genetic analysis revealed a 5-bp deletion in exon 4 of tubgcp3 (A), a 12-bp deletion combined with an 8-bp insertion in exon 7 of tubgcp4 (B), and a 7-bp deletion in exon 11 of tubgcp5 (C) in CRISPR/Cas9-generated mutants, resulting in frameshift mutations and prematurely truncated proteins. D WISH of cmyb showed a significantly reduced cmyb⁺ HSPCs in tubgcp3, tubgcp4, and tubgcp5 mutants at 5 dpf. The ratio in the lower right corner indicated embryos with representative expression of the corresponding genotype. Red arrows indicated signals in the region. Scale bars: 200 μm. E Quantification of cmyb⁺ cells in the CHT region of tubgcp3, tubgcp4, and tubgcp5 mutants and their respective siblings at 5 dpf. (mean ± SD; n > 10; Student’s t test and nonparametric Mann–Whitney test, ****p < 0.0001). Data were pooled from three independent experiments.

Expression Data

Gene:	myb
Fish:	tubgcp3^{zko902a/zko902a} tubgcp4^{zko3601A/zko3601A}
Anatomical Term:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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