Fig. 10

Yap1 activity is required between 10ss and 20ss for proper pLLP formation.

MIP of confocal Z-stacks showing the pLLP in 32 hpf embryos treated at 30% epiboly with DMSO (a) or increasing concentrations of K-975: 100 nM (b), 1 μM (c), 2 μM (d), 3 μM (e), and 5 μM (f). g Quantification of pLLP cell number at 32 hpf in the indicated groups (N = 1 independent replicate, except for DMSO and 1 μM K-975, N = 3). MIP of confocal Z-stacks showing the pLLP of 29 hpf embryos treated at 10ss (14 hpf) (h–k) or at 15ss (16.5 hpf) (l–o) with DMSO (h, l) or 1 μM (i, m), 5 μM (j, n), and 10 μM (k, o) K-975. p Quantification of pLLP cell number at 29 hpf in the indicated groups (N = 1 independent replicate). MIP of confocal Z-stacks showing the pLLP of 29 hpf embryos treated at 20ss (19 hpf) with DMSO (q) or 10 μM (r) and 20 μM (s) K-975. t Quantification of pLLP cell number at 29 hpf in the indicated groups (N = 1 independent replicate). MIP of confocal Z-stacks showing the pLLP of 29 hpf embryos treated from 10ss (14 hpf) to 20ss (19 hpf) with DMSO (u) or 1 μM K-975 (v). w Quantification of pLLP cell number at 29 hpf in the indicated groups (N = 1 independent replicate). Data are presented as mean ± SD. Unpaired t-tests (Mann–Whitney) were conducted. Scale bar: 20 μm (a-f, h-o, q-s, u, v).