FIGURE

Fig. 5

ID
ZDB-FIG-250310-13
Publication
Guo et al., 2024 - Wnt2bb signaling promotes pharyngeal chondrogenic precursor proliferation and chondrocyte maturation by activating Yap expression in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Wnt2bb controls pharyngeal chondrocyte maturation by activating β-catenin dependent signaling pathway. A and B: wnt2bb−/− embryos with a Tg(fli1:EGFP) background were treated with 10 μM BIO or 10 μM HLY78 from 28 hpf, and collected for Alcian blue staining (A) and live imaging (B) at 5 dpf, respectively. 3D reconstruction of chondrocytes (B) in ceratohyal cartilages was performed using Imaris software. The dotted lines and asterisks represent individual cells subjected to in-depth analysis via 3D reconstruction. C: The volume of individual chondrocytes in ceratohyal cartilages was quantified via Imaris (n = 7). ∗∗∗, P < 0.001; ns, not significant (student's t-test). WT, wild-type; m, Meckel's cartilage; ep, ethmoid plate; pq, palatoquadrate; hs, hyosymplectic; ch, ceratohyal; cbs, ceratobranchial cartilages; dpf, days post fertilization. Scale bars, 100 μm (A); 20 μm (B).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Journal of genetics and genomics = Yi chuan xue bao, , Guo, X., Yang, L., Wang, Y., Yuan, M., Zhang, W., He, X., Wang, Q., Wnt2bb signaling promotes pharyngeal chondrogenic precursor proliferation and chondrocyte maturation by activating Yap expression in zebrafish, , Copyright (2024) with permission from Elsevier. Full text @ J. Genet. Genomics