Fig. 7.

cx43 and axin2 expression are unchanged with Yap inhibition. Fins were amputated at 50% and treated with 20 µM Verteporfin at 72 hpa via injection. Fins were harvested 24 h later at 96 hpa. Amputation planes are denoted by white dotted line. (A) In situ hybridization was performed using antisense digoxygenin-labeled probe against cx43 to measure relative gene expression (n=4 for each treatment, with three biological replicates). (B) Gene expression was quantified through qPCR in both DMSO and Verteporfin treated fins. Graph shows a mean of three biological replicates fold difference and standard deviation (n=5 fins per replicate). A fold difference of 1 means no change from wild-type expression. The Student's t-test (two tailed, unpaired) was used to assess significance with a P value of 0.32. (C) In situ hybridization was performed using antisense digoxygenin-labeled probe against axin2 to measure relative gene expression (n=4 for each treatment, with three biological replicates). (D) Gene expression was quantified through qPCR in both DMSO and Verteporfin treated fins. Graph shows a mean of three biological replicates fold difference and standard deviation (n=5 fins per replicate). A fold difference of 1 means no change from wild-type expression. The Student's t-test (two tailed, unpaired) was used to assess significance with a P-value of 0.43. Scale bar: 100 µm.