FIGURE

Fig. 5

ID

ZDB-FIG-250219-13

Publication

Yang et al., 2024 - Manic Fringe promotes endothelial-to-mesenchymal transition mediated by the Notch signalling pathway during heart valve development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

MFNG promoted EndMT by upregulating the activity of Notch signalling pathway. a–d Transcriptional analysis revealed that HUVECs overexpressing MFNG exhibited increased expression of Notch pathway molecules such as HEY1 and NICD, along with elevated levels of EndMT-associated genes, including VIMENTIN. Conversely, CD31 expression was restored following DAPT treatment. GAPDH was utilized as an internal reference for these evaluations. e, f Western blot analysis, represented by the accompanying blots and quantitative data, confirmed these findings at the protein level, illustrating alterations in EndMT markers and Notch pathway molecules. β-actin served as the loading control. g, i Cell migration capabilities were assessed through wound healing and transwell assays. h Quantitative analysis of wound area healing was conducted using ImageJ software. j The quantification of cell migration ability was performed by counting cells per field. All data presented are the result of three independently repeated biological experiments

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Med.