FIGURE

Fig. 5

ID

ZDB-FIG-250204-5

Publication

Xiao et al., 2025 - Hsf1 is essential for proteotoxic stress response in smyd1b-deficient embryos and fish survival under heat shock

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Fig. 5

Coimmunoprecipitation (Co-IP) assay of Hsp70 binding with myosin chaperone Unc45b. (A) HEK293 cells were transfected with DNA plasmid expressing Myc-tagged Hsp70.3 or Hspa8 alone or co-transfected with plasmid expressing FLAG-tagged myosin chaperone UNC45b. Protein extract of the transfected cells was used for immunoprecipitation with anti-FLAG antibody and western analysis using anti-Myc and anti-FLAG antibodies. Co-IP results showed that Hsp70.3 and Hspa8 were coprecipitated with Unc45b. (B) To assess whether this coprecipitation correlated with the unregulated Hsp70s, the Co-IP assay was performed with two upregulated cytosolic Hsp70s (Hspa1b and Hsc70), and the mitochondria Hspa9 which was not upregulated in smyd1b mutant embryos. The Co-IP results showed that Hspa1b and Hsc70 were coprecipitated with Unc45b. In contrast, the mitochondrial Hspa9 that was not coprecipitated with Unc45b.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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