FIGURE

Fig. 4

ID

ZDB-FIG-250110-171

Publication

Singh et al., 2024 - Biallelic variants in CCN2 underlie an autosomal recessive kyphomelic dysplasia

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Fig. 4

Phenotypes in the ccn2a FO knockout zebrafish.

A Relative expression levels of the ccn2a mRNA in the controls (NT) and injectants (crispants). B Bright field image of a group of ccn2a crispants and matched control injectants from the same experiment to illustrate the extent of physical phenotypes. C Representative images of observed phenotypes as labeled, among the ccn2a crispants. D Quantification of the number of larvae showing the indicated phenotypes in each group. E Alcian blue stained images of wildtype and representative examples of ccn2a crispants, showing cartilage deformities. Cartilage elements labeled: m: Meckel’s cartilage; ch: ceratohyal; cb: ceratobranchial pairs. F Fold change in mRNA expression of select markers such as col2a1a (chondrocyte), sp7 (osteoblast), runx2a (early osteoblast) and rho1a (palatogenesis) in the ccn2a crispants as compared to the controls at 5dpf. Results from at least 5 independent experiments (pooled larvae, 30–50/experiment) are quantified.

Expression Data

Genes:	col2a1a rhoaa runx2a sp7
Fish:	WT WT + CRISPR10-ccn2a + CRISPR11-ccn2a
Knockdown Reagents:	CRISPR10-ccn2a CRISPR11-ccn2a
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR10-ccn2a + CRISPR11-ccn2a
Knockdown Reagents:	CRISPR10-ccn2a CRISPR11-ccn2a
Observed In:	caudal fin ceratobranchial cartilage ceratohyal cartilage cranial cartilage cartilage development heart Meckel's cartilage whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Eur. J. Hum. Genet.